Naftali Kaminski (@KaminskiMed )

Naftali Kaminski

Bio First Generation Physician-Scientist, & many other things. This account is Private - opinions and tweets do not represent any of the organizations I work with.
Location Yale School of Medicine
Tweets 21,7K
Followers 3,3K
Following 1,2K
Account created 09-07-2012 02:26:48
ID 630713215

iPhone : Recently added to the Atlas of Medical Foreign Bodies. Descriptions can be seen at website: flickr.com/photos/foreign…. Contributions appreciated; email to frbodies@gmail.com. Full details at tinyurl.com/yyx5jord

iPhone : Tata-lab_Duke Poss Lab Soderling Lab Cagla Eroglu Naftali Kaminski Darrell Kotton Daniel Tschumperlin James Kiley, PhD jon kropski Stijn De Langhe Gisli Jenkins Melanie Königshoff Oliver Rawlins Lab Marrah Lachowicz-Scroggins Martijn Nawijn Congratulations to this very important work Tata! I like your interpretation of the data and in particular the DNA damage aspect! As discussed earlier these experiments are highly complementary with our findings described in a recent pre-print: biorxiv.org/content/10.110…
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iPhone : Tata-lab_Duke Poss Lab Soderling Lab Cagla Eroglu Naftali Kaminski Darrell Kotton Daniel Tschumperlin James Kiley, PhD jon kropski Stijn De Langhe Gisli Jenkins Melanie Königshoff Oliver Rawlins Lab Marrah Lachowicz-Scroggins Martijn Nawijn Our single cell analysis identified fibrogenesis specific transient intermediate cells we termed Krt8+ alveolar differentiation intermediates (we agree with our reviewers that initially calling them progenitors was misleading) that are identical to PATS described here.
2/8

iPhone : Tata-lab_Duke Poss Lab Soderling Lab Cagla Eroglu Naftali Kaminski Darrell Kotton Daniel Tschumperlin James Kiley, PhD jon kropski Stijn De Langhe Gisli Jenkins Melanie Königshoff Oliver Rawlins Lab Marrah Lachowicz-Scroggins Martijn Nawijn Our single cell analysis identified fibrogenesis specific transient intermediate cells we termed Krt8+ alveolar differentiation intermediates (we agree with our reviewers that initially calling them progenitors was misleading) that are identical to PATS described here.
2/8

iPhone : Tata-lab_Duke Poss Lab Soderling Lab Cagla Eroglu Naftali Kaminski Darrell Kotton Daniel Tschumperlin James Kiley, PhD jon kropski Stijn De Langhe Gisli Jenkins Melanie K√∂nigshoff Oliver Rawlins Lab Marrah Lachowicz-Scroggins Martijn Nawijn ‚Ķ we assume highly complex regulatory niche signals to control the differentiation process while several aspects nevertheless happen when AEC2 `differentiate¬ī to AEC1 in highly artificial 2D in vitro cultures.
Exciting times ahead!!
8/8

iPhone : Tata-lab_Duke Poss Lab Soderling Lab Cagla Eroglu Naftali Kaminski Darrell Kotton Daniel Tschumperlin James Kiley, PhD jon kropski Stijn De Langhe Gisli Jenkins Melanie Königshoff Oliver Rawlins Lab Marrah Lachowicz-Scroggins Martijn Nawijn I am intrigued by your concept that the cell shape change itself may cause several of the observed gene expression changes as a consequence of stretching induced DNA damage (and possibly many other effects). This may help resolving the contradiction that on the one hand...
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iPhone : Tata-lab_Duke Poss Lab Soderling Lab Cagla Eroglu Naftali Kaminski Darrell Kotton Daniel Tschumperlin James Kiley, PhD jon kropski Stijn De Langhe Gisli Jenkins Melanie Königshoff Oliver Rawlins Lab Marrah Lachowicz-Scroggins Martijn Nawijn We have evidence that in the bleomycin model distinct stem cell lineages (AEC2 and Sox2+ airway) converge to Krt8+ ADI / PATS in alveolar regeneration. Thus, not lineage but either the injured alveolar niche or other factors determined transcriptional cell state.
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iPhone : Tata-lab_Duke Poss Lab Soderling Lab Cagla Eroglu Naftali Kaminski Darrell Kotton Daniel Tschumperlin James Kiley, PhD jon kropski Stijn De Langhe Gisli Jenkins Melanie Königshoff Oliver Rawlins Lab Marrah Lachowicz-Scroggins Martijn Nawijn Thus, the availability of these factors (we validated Areg, Hbegf and Itgb6 on protein level) during the fibrogenic phase is likely dependent on the Krt8+ ADI / PATS cell state, while its transient nature elegantly enables the system to temporally limit their expression.
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iPhone : Tata-lab_Duke Poss Lab Soderling Lab Cagla Eroglu Naftali Kaminski Darrell Kotton Daniel Tschumperlin James Kiley, PhD jon kropski Stijn De Langhe Gisli Jenkins Melanie K√∂nigshoff Oliver Rawlins Lab Marrah Lachowicz-Scroggins Martijn Nawijn Our analysis identified similar pathways as you described, including the p53 pathway. The signature includes genes such as Ctgf, Itgb6, Areg, Hbegf, Edn1 and Lgals3, all of which are antifibrotic targets that have been tested in pre‚Äźclinical and clinical studies.
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